Gene: Lysostaphin gene from Staphylococcus simulans, Genbank accession numbers M15686 and I00376
Expression organism: E. coli
Activity assay: Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus 8325-4 at pH 8.0, 30° C (AMBI Protocol TP-031). Specific activity is measured relative to an AMBI lysostaphin standard with a defined activity of 4,000 U/mg
Storage conditions:
Long-term: Store in sealed container with desiccant at -20° C.

Solution: Stable for at least 6 months when stored at 4°C as 10 mg/ml solution in 20 mM sodium acetate, pH 4.5, in a polypropylene container.
Method of analysis: Reverse phase HPLC monitored at 210 nm (AMBI Protocol TP-112.M)
Peptide purity: >90% (reverse phase HPLC)
mg protein/mg solid: >0.9 (Absorbance at 280 nm; extinction coefficient = 2.02 AU/mg/ml)
Additives: None
Specific activity: >3,000 units/mg (Turbidometric assay AMBI Protocol TP-031)
Physical form: Lyophilized powder
Shelf life: <1% loss of activity per year, when stored dry at -80° C
Batch Information  
Lot Number: GDV 1
Specific activity: 3552 units/mg
Peptide purity: 93.4% (HPLC, % of peak area)
Recsei, P.A. (June 5, 1990) U.S. Pat. # 4931390.
Recsei, P.A., Gruss, A.D., and Novick, R.P. (1987) Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Nat. Acad. Sci., U.S.A. 84: 1127-1131.
Kline, S.A., J. de la Harpe, and P. Blackburn. (1994). A colorimetric microtitre plate assay for lysostaphin using a hexaglycine substrate. Anal. Bioch. 217, 329-331.
Schindler, C.A. and V.T. Schuhardt. (1964) Lysostaphin: a new bacteriolytic agent for the stapylococci.Proc. Nat. Acad. Sci. U.S.A. 51: 414-421.
Novick, R. P. (1991). Methods in Enzymology 204:587-636
Goering , R.V and M. A. Winters. (1992). Rapid method for epidemiological evaluation of Gram-positive cocci by field inversion gel electrophoresis. J. Clin. Microbiol. 30:577-580
Bannerman, T. L., G. A. Hancock, F. C. Tenover and J. M. Miller. (1995). Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. J. Clinical Microbiol. 33:551-555